Sanger workflow

The Sanger workflow has 2 protocols:

  1. PCR Mix :
  • Prepare PCR aliquots : Derivation step
  • Plate design : Link location step to set aliquot position on plate
  • PCR mix : Link reagents step with usage tracking
  1. Sequencing mix:
  • Prepare sequencing aliquots: Derivation step
  • Plate design : Link location
  • Sequencing mix : Link reagents step with usage tracking

Usage tracking options are not shared with SlimsShare. Then Link reagents steps should be updated as shown on following screenshot:

Sequencing mix is usually fixed and should then be set on the Prepare Sequencing aliquots step definition.

A Sanger requestable is created.

Following custom fields are created :

  • Master mix on Protocol step
  • Master mix, Master mix dead volume and Master mix recipe on Protocol run step.
  • Primer pairs on Request

PRELIMINARY CONDITIONS

Sanger set up workflow should be imported first (see its preliminary conditions)

HOW TO USE

The requests should be set with a list of selected primer pairs : this can be done in the ORDER module only.

This workflow is designed to work with a set of 3 SLIMS GATE flows:

  • 1 flow to create the PCR products
  • 1 flow to create the Sequencing products
  • 1 flow to compute reagents usage

Please request the flows to an engineer.

(SLIMS GATE plugin exists)