This package includes content types:

  • PCR product,
  • Sequencing product,
  • Primer
  • Reagent

and Reference data:

  • PCR program
  • Primer references
  • Primer pair reference
  • Ingredient category, Reagent type, Ingredient

Additional custom fields on SOP for master mix definition.

PCR product has additional custom fields :

  • Primer 1 for Forward primer content
  • Primer 2 for Reverse primer content
  • Primer pair reference for Primer pair reference
  • Mix recipe for PCR mix Recipe
  • Volume

Sequencing product has additional custom fields :

  • Primer 1 for sequencing primer content
  • Primer pair reference for Primer pair reference
  • Mix Recipe for Sequencing mix Recipe

Primer has additional custom fields:

  • Primer Reference for primer Reference data record
  • Rehydration Author, Volume and Date
  • Purification
  • Reception Date
  • Scale
  • State

Reagent has additional custom fields:

  • Reagent type
  • Volume

PCR program has no custom fields, only a Name

Primer Reference has additional custom fields :

  • Sequence
  • Size (bp)
  • Tm (melting temperature)
  • 5′, 3′ and internal modification

Primer pair reference has additional custom fields :

  • Gene
  • Exon
  • Forward primer
  • Reverse primer
  • Amplicon size
  • SOP mix (=PCR mix recipe)
  • PCR program
  • Sequencing primers

Ingredient category has no custom fields.
Following records should exist :

  • Sample
  • PCR primer pair
  • Sequencing primer
  • Reagents Mix 1
  • Reagents Mix 2
  • MLPA probe

Reagent type has no custom field, only a name. It is used to detail the type of Reagent.

Ingredient has additional custom fields :

  • Name (is automatically computed)
  • Ingredient category
  • Reagent type
  • Volume / sample
  • position (for recipe display)
  • option “is optional”
  • option “track usage”

SOP has additional custom fields :

  • option “Contains master mix definition”
  • Mix ingredients if above is checked

Preliminary imports :

  1. Install SI units from Store
  2. Install Molar concentration unit from Store
  3. Import NGSLabManagerRole
  4. Install Miseq package
  5. Install Generic Content Types from Store