qRT-PCR Workflow

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qRT-PCR Workflow

Quantitative reverse transcription PCR (RT-qPCR) is considered to be the most powerful, sensitive, and quantitative assay for the detection of RNA levels. It is frequently used in the expression analysis of single or multiple genes, and expression patterns for identifying infections and diseases. qRT-PCR is used when the starting material is RNA. In this method, RNA is first transcribed into complementary DNA (cDNA) by reverse transcriptase from total RNA or messenger RNA (mRNA). The cDNA is then used as the template for the qPCR reaction.

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Description

Quantitative reverse transcription PCR (RT-qPCR) is considered to be the most powerful, sensitive, and quantitative assay for the detection of RNA levels. It is frequently used in the expression analysis of single or multiple genes, and expression patterns for identifying infections and diseases. qRT-PCR is used when the starting material is RNA. In this method, RNA is first transcribed into complementary DNA (cDNA) by reverse transcriptase from total RNA or messenger RNA (mRNA). The cDNA is then used as the template for the qPCR reaction.

Additional information

slims_version

VERSION_6_1, VERSION_6_2

slimsshare_package_id

115, 65

packages

VERSION_6_3:182, VERSION_6_4:258