Quantitative reverse transcription PCR (RT-qPCR) is considered to be the most powerful, sensitive, and quantitative assay for the detection of RNA levels. It is frequently used in the expression analysis of single or multiple genes, and expression patterns for identifying infections and diseases. qRT-PCR is used when the starting material is RNA. In this method, RNA is first transcribed into complementary DNA (cDNA) by reverse transcriptase from total RNA or messenger RNA (mRNA). The cDNA is then used as the template for the qPCR reaction.
qRT-PCR Workflow
Description
Quantitative reverse transcription PCR (RT-qPCR) is considered to be the most powerful, sensitive, and quantitative assay for the detection of RNA levels. It is frequently used in the expression analysis of single or multiple genes, and expression patterns for identifying infections and diseases. qRT-PCR is used when the starting material is RNA. In this method, RNA is first transcribed into complementary DNA (cDNA) by reverse transcriptase from total RNA or messenger RNA (mRNA). The cDNA is then used as the template for the qPCR reaction.
Additional information
| slims_version | VERSION_6_1, VERSION_6_2 |
|---|---|
| slimsshare_package_id | 115, 65 |
| packages | VERSION_6_3:182, VERSION_6_4:258 |
