This package contains an SOP for:
Agilent – Agilent SureGuide Cas9 Nuclease Kit
Cas9 (CRISPR associated protein 9) is an RNA- guided DNA nuclease associated with Type II bacterial CRISPR immunity systems. Cas9 is widely used to induce site- specific double strand breaks in DNA for multiple applications, many of which have yet to be developed. The guide RNA (gRNA) structure that binds and directs Cas9 is a hybrid of CRISPR RNA, containing homologous sequence to the cleavage target, and a trans- activating tracrRNA.