This package contains an SOP for:
Agilent – Absolutely RNA miRNA Kit
The Absolutely RNA miRNA Kit provides a rapid method for purification of high-quality, miRNA-containing total RNA from cultured cells or small samples of tissue. The Absolutely RNA miRNA Kit method employs a spin cup with a matrix that binds cellular RNA, with a very low size cutoff, in the presence of ethanol and a chaotropic salt. Both miRNA and mRNA species are quantitatively recovered using this method, allowing gene expression studies of both miRNA and mRNA levels from the same sample using QRT-PCR or microarray analysis.